Mixed glycosidase pretreatment reduces nonspecific binding of antibodies to frozen tissue sections.
نویسندگان
چکیده
منابع مشابه
Accuracy of Frozen Sections
Background and Objective: Intraoperative consultation by frozen section is a high – risk procedure with important consequences. Therefore, it is critical to determine efficiency of frozen section performance periodically. This study was performed to determine the accuracy of frozen section in Urmia University of Medical Sciences. Materials & Methods:Inthis cross sectional study, we compared th...
متن کاملMixed Agglutination with Tissue Sections
The mixed agglutination procedure was applied to tissue sections. Microtome sections of bovine tissue were placed on coverglasses, fixed by acetone or formalin, and incubated with various dilutions of rabbit antisera. The binding of antibodies to the tissue sections was detected by the addition of an indicator system composed of sheep erythrocytes sensitized by subagglutinating doses of the cor...
متن کاملFunctional Significance of Calcium Binding to Tissue-Nonspecific Alkaline Phosphatase
The conserved active site of alkaline phosphatases (AP) contains catalytically important Zn2+ (M1 and M2) and Mg2+-sites (M3) and a fourth peripheral Ca2+ site (M4) of unknown significance. We have studied Ca2+ binding to M1-4 of tissue-nonspecific AP (TNAP), an enzyme crucial for skeletal mineralization, using recombinant TNAP and a series of M4 mutants. Ca2+ could substitute for Mg2+ at M3, w...
متن کاملStudy of vitrified, unstained frozen tissue sections by cryoimmunoelectron microscopy.
We describe the development and application of a novel approach to high-resolution ultrastructural analysis of cells and tissues. It is based on the preparation of ultrathin frozen sections of fixed tissues, rinsing of the sections, followed by their embedding on the grid in a layer of vitrified ice, and direct observation with a cryoelectron microscope. Examination of smooth muscle, kidney and...
متن کاملaccuracy of frozen sections
background and objective: intraoperative consultation by frozen section is a high – risk procedure with important consequences. therefore, it is critical to determine efficiency of frozen section performance periodically. this study was performed to determine the accuracy of frozen section in urmia university of medical sciences. materials & methods:inthis cross sectional study, we compare...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1985
ISSN: 0022-1554,1551-5044
DOI: 10.1177/33.7.3891844